The portrait shows Izabela Habsburg, daughter of Joanna Szalona and Filip Piekny. In Puławy, the painting was considered to be a portrait of Anna Boleyn. It was probably donated to the Gothic House in the Czartoryski residence in Puławy by Wincenty Krasiński. Previously, the painting was owned by the niece of the last king before the First Partition of Poland, Stanisław August Poniatowski.