The biblical story of Susannah tells how she was left by her maids to bathe in the garden. Here she was spied upon by two elders of the community who threatened to accuse her of adultery if she did not yield to their demands. The painting combines three episodes from the story: in the centre, Susannah’s arrival in the garden; on the right, the departure of her maids; and on the left, her confrontation with the elders. The building on the right is typical of the architecture of 15th century Florence. The painting almost certainly belonged to a series illustrating the story of Susannah, which would have formed a decorative frieze.