Augusto Napoleone Berlese was an Italian botanist and mycologist. He was the brother of entomologist Antonio Berlese 1863–1927, with whom he founded the journal Rivista di patologia vegetale in 1892.
He studied natural sciences at the University of Padua, where following graduation, he worked for several years as a botanical assistant. Later on, he taught classes at the viticulture school in Avellino, and at the universities of Camerino and Sassari. In 1901 he was appointed professor of phytopathology at the agricultural college in Milan.
The mycological genus Berlesiella was named in his honor by Pier Andrea Saccardo.