Niccolò di Segna was an Italian painter from Siena. His activity is documented starting from 1331.
Influenced by Duccio di Buoninsegna and Simone Martini, he was an exponent of the Sienese School. He collaborated with Pietro Lorenzetti to the frescoes in Santa Maria dei Servi and painted. Other works by him can be found in the Pinacoteca Nazionale at Siena, in the Cathedral of Sansepolcro, the Diocesan Museum of Cortona and other collections in Italy and abroad.