The painting depicts Pope Leo X (Giovanni de' Medici, 1475-1521), son of Lorenzo il Magnifico, with Giulio de' Medici (1478-1534), future Pope Clement VII to the left and Luigi de Rossi (1474-1519), his cousin, to the right. The painting was sent to Florence in 1518 for the wedding of Lorenzo de' Medici, Duke of Urbino, and Maddalena de la Tour d'Auvergne. It was exhibited in the tribune from 1589.
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