Little Missenden is a village and civil parish on the River Misbourne in Buckinghamshire, England. It is in the Chiltern Hills, about 3 miles southeast of Great Missenden and 3 miles west of Amersham. The village lies on the River Misbourne in the Misbourne valley.
The parish includes the villages of Holmer Green, Hyde Heath and Little Kingshill, and the hamlets of Beamond End, Brays Green, Mop End and Spurlands End. The 2011 Census recorded the population of the ward of Little Missenden, which includes Hyde Heath and Little Kingshill, as 2,234, albeit those other two villages in the ward are each larger than Little Missenden itself. The population of the entire parish was estimated as 6,490 in 2017, reflecting the inclusion of the much larger Holmer Green within the parish.
The main London – Aylesbury road used to run through the centre of Little Missenden and past the two pubs – The Red Lion and The Crown. Early in the 19th century a new by-pass road was built to the north and this now forms part of the modern A413 road.