Ju Lian learnt painting under his cousin Ju Chao. Entitled "A Hundred Flowers", this long handscroll measures over six hundred centimetres and is one of the masterpieces among his extant works. This monumental work is rendered with a comprehensive composition which is exquisitely executed and meticulously coloured with vividly depicted flowers. The elegant bird-and-flower tradition, which prospered in the Song Painting Academy, was refined by Chen Chun (1483 - 1544) of the Ming dynasty and further improved by Ju Lian's special techniques - the zhuangfen and zhuangshui, or "water and pigment infusion" method. Based on the traditional "boneless" method (painting without outlines), Ju deposited water and pigment onto the wet painted surface. It then dried to form a clearly defined boundary, similar to the effect of the outline method. This painting is one of the best examples of the aforesaid techniques.