The Ambassadors' is a painting full of clues, symbols, and mystery.
At first glance, the picture celebrates two wealthy, educated and powerful young men.
On the left is Jean de Dinteville, aged 29, the French ambassador to England in 1533.
Hans Holbein the Younger
To the right stands his friend, Georges de Selve, 25, the Bishop of Lavaur, who acted as ambassador to the Emperor, the Venetian Republic, and the Holy See.
Hans Holbein the Younger
Making sense of our place in the universe
On the upper shelf there's a celestial globe, a portable sundial, and other instruments for understanding the heavens and measuring time.
Some details could be seen as references to religious tensions at the time.
The lute was among the most popular instruments of the 16th century. The broken string could symbolise religious discord...
...while this Lutheran hymn book might suggest a plea for Christian harmony.
Hans Holbein the Younger
But perhaps the greatest mystery is the strange object that appears to have been smeared across the front of the painting.
Visit the painting at the National Gallery in London, walk to the right and look back across the surface. All of a sudden, you see that it's a skull. Some say that the skull is a message about how close we are to death in daily life.
Hidden in the upper left-hand corner of the canvas is Christ on the crucifix.
For Holbein and his contemporaries, Christ’s presence on the canvas meant only one thing - a symbol of salvation.