This is the central fresco of the Room of Night and Day, carried out by Jacopo Vignali in 1525. In an octagonal shape, surrounded by the Buonarroti’s coats of arms, the Eternal Father, characterized by a vast blanket and by a flowing white berad, is represented in the moment of separating the light of the day from the darkness of the night, characterized as two curtains moved toward the ends.