This painting which represents the test of Abraham's faith in which he is asked by God to sacrifice his only child, a child he had at a very advanced age, is the only painting by Caravaggio with a landscape in the background. The Sacrifice of Isaac is cited in the inventories from Casa Barberini in Rome and then from 1812 to the end of the 19th century it was part of the Colonna di Sciarra collection. It was donated to the Uffizi by John Fairfax Murray in 1917.
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