Acquoy is a village in the Dutch province of Gelderland. It is a part of the municipality of West Betuwe, and lies about 12 km east of Gorinchem.
In 2001, the village of Acquoy had 278 inhabitants. The built-up area of the village was 0.079 km², and contained 115 residences.
The statistical area "Acquoy", which also can include the peripheral parts of the village, as well as the surrounding countryside, has a population of around 520. In January 2006 Acquoy had 617 inhabitants.
According to popular legend a "Jan van Arkel" was the first to have settled the village of Acquoy, when he returned from the crusades in 1133. However, this is highly unlikely, because there were no crusades in the period around 1133. The first crusade was from 1096–99 and the second one was from 1147-49. Some sources report that the Jan van Arkel mentioned was in fact Jan I van Arkel. However, he lived a century later and had the nickname "de Sterke", which means "the strong".
In 1305 Acquoy is mentioned as part of the property of the lords of Voorne. In 1364 Catherina van Voornenburgh rented her house together with the stronghold of Acquoy to Otto van Arkel for a period of 10 years and later he bought it.