Domenico di Michelino was an Italian Renaissance painter who was born and died in Florence. His real name was Domenico di Francesco. The patronymic "di Michelino" was adopted in honor of his teacher, the cassone painter Michelino di Benedetto, by whom no works have been identified. Giorgio Vasari reports that Domenico was also a pupil of Fra Angelico, whose influence is reflected in many of Domenico's paintings along with that of Filippo Lippi and Pesellino.
Domenico enrolled in the Florentine painters' confraternity, the Compagnia di San Luca, by 1442. Two years later he joined the Arte dei Medici e Speziali, the Florentine painters' guild. He had a workshop in the Via delle Terme, Florence, which he shared with Domenico di Zanobi.
His earliest extant work is a processional banner for the Ospedale degli Innocenti, Florence, in which the Virgin is shown protecting the martyred innocents beneath her mantle. Commissioned in 1440 and completed in 1446, the picture was entirely repainted in the sixteenth century by Michele Tosini.
In 1449-50 Domenico painted the chapel of Saint Leonard in the church of Santa Maria a Peretola in the outskirts of Florence.