Mespelbrunn is a community in the Aschaffenburg district in the Regierungsbezirk of Lower Franconia in Bavaria, Germany and a member of the Verwaltungsgemeinschaft of Mespelbrunn, whose seat is in Heimbuchenthal.
It is the site of Mespelbrunn Castle, one of the most famous landmarks of the Spessart region, and of the Wallfahrtskirche Hessenthal, for more than 700 years the destination of a Christian pilgrimage.