Santa Ana National Wildlife Refuge is a 2,088-acre National Wildlife Refuge situated along the banks of the Rio Grande, south of Alamo in the Lower Rio Grande Valley, in Hidalgo County, South Texas.
The wildlife refuge was established for the protection of migratory birds in 1943. Its unique location is at the meeting of different climates and habitats: subtropical wetlands, Chihuahuan Desert, Gulf Coast, and Great Plains. Its riparian location has developed a reputation for diverse birding.
Due to its location near the Mexico–United States border, this wildlife refuge is threatened by the Trump wall.