Orchidaceae963

Developing a bait set to sequence 963 genes across orchids

Calopogon tuberosa flower (3/27/2020) by Jeff TalbertSoutheastern Center for Conservation at the Atlanta Botanical Garden

Orchid Diversity

Orchids are one of the most speciose group of plants on Earth. There are nearly 27,000 recognized species of orchids, with new species being described every year.

Gastrochilus bellinus (2018-03-16) by Lauren EsermanSoutheastern Center for Conservation at the Atlanta Botanical Garden

Beauty comes at a cost

Unfortunately, many orchid species face extinction in the wild. The main threats are poaching for the illegal orchid trade, habitat destruction, and disease. Scientists today are developing tools to help combat orchid extinction.

Amber Rittgers in the Conservation Genetics Lab (2019-07-29) by Bo ShellSoutheastern Center for Conservation at the Atlanta Botanical Garden

Orchidaceae963

Scientists at the Atlanta Botanical Garden, in collaboration with the University of Georgia and the University of Missouri-Columbia, have developed at toolkit that allows researchers to sequence hundreds of genes from a single DNA sample. 

Platanthera integrilabia (2018-08-23) by Melina Lozano-DuranSoutheastern Center for Conservation at the Atlanta Botanical Garden

21st Century Orchid Genetics

This toolkit, called the Orchidaceae963, allows for advances in orchid phylogenetics, population genetics, and molecular identification.

Cypripedium kentuckiense (2018-05-14) by Emily CoffeySoutheastern Center for Conservation at the Atlanta Botanical Garden

Where we can improve

The poorest performance of the Orchidaceae963 is in the Cypripedioideae and Orchidoideae subfamilies, which are the slipper orchids and other terrestrial orchids. We are digging into the data to understand why and where we can improve performance for these species.

Isotria medeoloides (2019-05-06) by Emily CoffeySoutheastern Center for Conservation at the Atlanta Botanical Garden

Future work

We plan to keep improving. The next steps involve improving performance for the terrestrial and slipper orchids, further testing on the Vanilla subfamily, and testing the utility for population genomics.

We are excited to see how this toolkit is used in the future!!

Catasetum planiceps (2018-03-16) by Lauren EsermanSoutheastern Center for Conservation at the Atlanta Botanical Garden

Read the paper!

Check out the open access publication of the Orchidaceae963 toolkit here in Applications in Plant Sciences

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